3
Revision Date: Feb.11, 2010
Injecting
Necropsy
Animal handling
Sample collection
3. ATPs-L known or reasonably expected to be present in the laboratory.
Please refer to appendix D of the Cal-OSHA Title 8 Standard 5199
(https://www.dir.ca.gov/Title8/5199.html) and list the ATPs in use in the laboratory:
Name Form (culture, infected tissue,
environmental sample, etc)
Use additional pages if necessary
4. Safe handling practices:
a. Practices for handling samples and specimens will follow the standards described in the
latest edition of the Centers for Disease Control (CDC) Biosafety in Microbiological and
Biomedical Laboratories (BMBL). These are Standard Practices to be used by all
laboratories that work with ATP’s-L, to be supplemented by other practices as appropriate.
i. Access to the laboratory is controlled by written institutional and investigator policy.
ii. Persons must wash their hands after working with potentially hazardous materials,
after removing gloves, and before leaving the laboratory.
iii. Eating, drinking, smoking, handling contact lenses, or applying cosmetics is not
permitted in the laboratory. Food must be stored in areas outside of the laboratory
designed for this purpose.
iv. Mechanical pipetting devices are to be used at all times (no mouth pipetting
allowed).
v. Sharps including razor blades, scalpels, and hypodermic needles and other sharp
instruments are limited to use only when there is no alternative.
a. Disposable sharps must be placed in hard-walled sharps waste containers
designed and approved specifically for that purpose.
b. Non-disposable sharps must be transported to the autoclave in a hard-
walled container for decontamination before further processing.
c. Plasticware should be substituted for glassware wherever possible.
d. Broken glass must be picked up with broom or brush and dustpan, tongs, or
forceps and disposed to a container approved for that use.
vi. Perform all procedures in a manner that minimizes splashes or aerosols.
vii. Perform all aerosol-generating procedures in a containment device such as a
biological safety cabinet or a centrifuge with sealed rotors.
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